Article

Detection of Subgroup III Geminivirus Isolates in Leaf Extracts by Degenerate Primers and Polymerase Chain Reaction

1996-01-01

Abstract excerpt

The DNA of several monopartite and bipartite whitefly-transmitted (WFT) geminiviruses was amplified from a viral template present in infected leaves after either direct addition of clarified plant extracts to an otherwise complete polymerase chain reaction (PCR) mix or after immobilization of template to microfuge tubes. A degenerate primer pair was designed to specifically target the middle or 'core' region of th...

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Literature Corpus work: 5ec43c1c-b41d-5d23-b5e2-3ec79ba701d3
DOI: 10.1094/phyto-86-1288

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