Article
Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription-polymerase chain reaction.
Virology journal - 22 Nov 2016
Zhang Xiaoyan, Peng Yanmei, Wang Ying, Zhang Zongying, Li Dawei, Yu Jialin, Han Chenggui
Abstract excerpt
BACKGROUND: Brassica yellows virus (BrYV), proposed to be a new polerovirus species, three distinct genotypes (BrYV-A, BrYV-B and BrYV-C) have been described. This study was to develop a simple, rapid, sensitive, cost-effective method for simultaneous detection and differentiation of three genotypes of BrYV. RESULTS: In this study, a multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was...
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