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A highly sensitive multiplex lateral flow immunoassay for simultaneous detection of Listeria monocytogenes, Salmonella Typhimurium and Escherichia coli O157:H7

2022-09-27

Abstract excerpt

In this study, a sensitive, fast and reliable multiplex lateral flow immunoassay based on multiple PCR and gold nanoparticles (AuNPs) was developed. Genomic DNA of Listeria monocytogenes , Salmonella Typhimurium and Escherichia coli O157:H7 was extracted by a simple boiling method. Three pairs of primers were designed and labeled according to specific gene fragment of the three strains for multiple PCR. The PCR pr...

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Literature Corpus work
5bb93624-b67a-5db4-ab64-1f14255fe506
DOI
10.21203/rs.3.rs-2073305/v1
Open publication

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