RNA-seq: primary cells, cell lines and heat stress

Abstract excerpt

Transcriptome analysis by RNA-seq has emerged as a high-throughput, cost-effective means to evaluate the expression pattern of genes in organisms. Unlike other methods, such as microarrays or quantitative PCR, RNA-seq is a target free method that permits analysis of essentially any RNA that can be amplified from a cell or tissue. At its most basic, RNA-seq can determine individual gene expression levels by countin...

Topics

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• Gene Expression Profiling
• Gene Expression Regulation
• Heat-Shock Proteins
• High-Throughput Nucleotide Sequencing
• Molecular Chaperones
• RNA Splicing
• Sequence Analysis, RNA
• Workflow

Identifiers and source

Literature Corpus work

5b8ca354-0c8d-56ea-90c1-d18843c818e7

DOI

10.1101/013979