Article

Collateral fitness effects of mutation are not commonly caused by protein misfolding

2025-09-14

Abstract excerpt

Mutations in coding sequences are often assumed to harm cells by destabilizing proteins and creating toxic misfolded species. Here we directly test how fitness scales with predicted folding stability. Using deep mutational scanning of a gratuitously expressed protein in S. cerevisiae (≈2,000 YFP single-amino-acid variants) and meta-analyses of seven additional scans of gratuitous proteins in yeast and E. coli ,...

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Literature Corpus work: 57a9fb0d-3887-5a39-b2da-f91327c2fff2
DOI: 10.1101/2025.09.12.675869

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