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Immunization for generation of hybridoma antibodies specifically reacting with plants infected with a mycoplasmalike organism (MLO) and their use in detection of MLO antigens

1990-10-01

Abstract excerpt

Employing a procedure by using mice neonatally injected with nontarget antigens present in immunogen preparation before immunization, mouse hybridomas that secreted antibodies to a mycoplasmalike organism (MLO) isolated from tomato plants with symptoms of big bud disease were produced. Neonatal mice were injected with extracts from normal host plant tissue on days 1 and 7. When mice were 8 wk old, they were immuni...

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Literature Corpus work
3bae6a03-8082-52ad-aad2-3ef37c89061d
DOI
10.1094/phyto-80-946
Open publication

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