Article

Characterization and molecular analysis of transgenic plants obtained by microprotoplast fusion in sunflower.

2000-12-01

Abstract excerpt

Asymmetric somatic hybrid (ASH) plants were obtained by PEG-mediated mass fusion of microprotoplasts from perennial Helianthus species and hypocotyl protoplasts of Helianthus annuus. The formation of micronuclei in perennial sunflower cell cultures was induced, at early log phase, by addition of the herbicides amiprophos-methyl or oryzalin. Sub-diploid microprotoplasts were isolated by high-speed centrifugation an...

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Literature Corpus work: 3af4a374-ba82-594a-9c43-347d85bcbcbc
DOI: 10.1007/s001220051604

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