Article

One-step RNA extraction for RT-qPCR detection of 2019-nCoV

2020-04-05

Abstract excerpt

<h4>ABSTRACT</h4> The global outbreak of coronavirus disease 2019 (COVID-19) has placed an unprecedented burden on healthcare systems as the virus spread from the initial 27 reported cases in the city of Wuhan, China to a global pandemic in under three month[1]. Resources essential to monitoring virus transmission have been challenged with a demand for expanded surveillance. The CDC 2019-nCoV Real-Time Diagnostic...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 3999225a-2a70-5615-947e-c79257fcc0f7
DOI: 10.1101/2020.04.02.022384

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

One-step RNA extraction for RT-qPCR detection of 2019-nCoVDOI 10.1101/2020.04.02.022384Read

A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCRSemantic proximity Read

Classic RNA extraction is efficient for subsequent detection of SARS-CoV-2 by RT-PCR in nasopharyngeal swab samplesSemantic proximity Read

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extractionSemantic proximity Read

Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCRSemantic proximity Read

Comparison of RNA extraction methods for the detection of SARS-CoV-2 by RT-PCRSemantic proximity Read

A Novel Two-Step, Direct-to-PCR Method for Virus Detection off Swabs using Human Coronavirus 229ESemantic proximity Read

Evaluation of RT-LAMP and Dry Swab RNA Extraction Free Method for Detection of SARS-CoV-2 InfectionSemantic proximity Read

Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocyclerSemantic proximity Read

Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis BufferSemantic proximity Read

Extraction-free protocol combining Proteinase K and heat inactivation for detection of SARS-CoV-2 by RT-qPCRSemantic proximity Read

Analysis of the initial lot of the CDC 2019-Novel Coronavirus (2019-nCoV) real-time RT-PCR diagnostic panelSemantic proximity Read

SARS-CoV-2 detection by direct rRT-PCR without RNA extractionSemantic proximity Read