Article

The Early History of the TYH Medium for<i>in vitro</i>Fertilization of Mouse Ova

2016-05-01

Abstract excerpt

The TYH medium was first reported as a medium for in vitro fertilization (IVF) of mouse eggs with epididymal spermatozoa, by Toyoda, Yokoyama and Hosi, in 1971. It was a modified Krebs-Ringer bicarbonate solution, supplemented with glucose, Na-pyruvate, antibiotics and bovine serum albumin. In TYH medium, almost all eggs are fertilized within 1 h, if the spermatozoa are pre-incubated for 2 h before insemination, w...

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Literature Corpus work: 33eaf856-fc7d-5874-bfc2-4ab7a47615a0
DOI: 10.1274/jmor.33.3

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