Article

Functional Genomics via Zebrafish CRISPR Reveals Pathogenic Landscape of 16 Candidate Genes in Hemifacial Microsomia

2025-09-26

Abstract excerpt

<title>Abstract</title> <p>Background: Hemifacial Microsomia (HFM) is a genetically complex craniofacial disorder. While GWAS and family studies have identified multiple candidate genes, functional validation rates remain low (<10%). <h4>Methods:</h4> We established a high-throughput zebrafish CRISPR-Cas9 platform to functionally validate 16 prioritized genes (12 literature-derived, 4 bioinformatically predicted)...

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Literature Corpus work: 32ba4de3-8b51-5c37-82f2-282373ca9a65
DOI: 10.21203/rs.3.rs-7317799/v1

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