Article

Development of a high-throughput barrier function assay using primary human gut organoids

2022-12-19

Abstract excerpt

Disruptions in the gut epithelial barrier can lead to the development of chronic indications such as inflammatory bowel disease (IBD). Historically, barrier function has been assessed in cancer cell lines, which do not contain all human intestinal cell types, leading to poor translatability. To bridge this gap, we adapted human primary gut organoids grown as monolayers to assess barrier function. In this work we d...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 2f08f454-44dd-587f-9f08-a3f44bd2417f
DOI: 10.1101/2022.12.16.520780

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Development of a high-throughput barrier function assay using primary human gut organoidsDOI 10.1101/2022.12.16.520780Read

Establishment of a 96-well transwell system using primary human gut organoids to capture multiple quantitative pathway readoutsSemantic proximity Read

Molecular and Functional Characterization of Human Intestinal Organoids and Monolayers for Modeling Epithelial BarrierSemantic proximity Read

Understanding disruption of the gut barrier during inflammation: Should we abandon traditional epithelial cell lines and switch to intestinal organoids?Semantic proximity Read

Generation of human colon organoids from healthy and inflammatory bowel disease mucosaSemantic proximity Read

The differentiation state of small intestinal organoid models influences prediction of drug-induced toxicitySemantic proximity Read

Organoids in gastrointestinal diseases: from bench to clinicSemantic proximity Read

Epithelial organoid cultures from patients with ulcerative colitis and Crohn's disease: a truly long-term model to study the molecular basis for inflammatory bowel disease?Semantic proximity Read

Colorectal Organoids: Models, Imaging, Omics, Therapy, Immunology, and EthicsSemantic proximity Read

Characterization and optimization of variability in a human colonic epithelium culture modelSemantic proximity Read

Development and Characterization of a Human and Mouse Intestinal Epithelial Cell Monolayer PlatformSemantic proximity Read

Organoid-derived intestinal epithelial cells are a suitable model for preclinical toxicology and pharmacokinetic studiesSemantic proximity Read

Gut organoids: mini-tissues in culture to study intestinal physiology and diseaseSemantic proximity Read