Article

Purification and characterization of an endopolygalacturonase from the rough lemon pathotype of Alternaria alternata, the cause of citrus brown spot disease

1997-09-01

Abstract excerpt

Alternaria alternata rough lemon pathotype (AC325) produced polygalacturonase in liquid culture containing 1% pectin. Synthesis of the polygalacturonase was repressed by the addition of 5% sucrose to the culture. The enzyme was purified about 31-fold from the filtrates in four steps: ammonium sulphate precipitation; CM-Sepharose; cation exchange HPLC; and gel filtration HPLC. The isolated polygalacturonase acted i...

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Literature Corpus work: 2926b0f8-9e4b-5726-bd8c-10cddd9651b0
DOI: 10.1006/pmpp.1997.0106

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