Article

Fragment end motif analysis to distinguish pathogens from contaminants in enriched plasma microbial DNA

2025-11-07

Abstract excerpt

<h4>Introduction</h4> Despite its promise, accuracy of microbial cell-free DNA (mDNA) in plasma as a diagnostic tool is hindered by its low abundance and process contaminants. We have previously shown that combining size selection with single-stranded DNA (ssDNA) library preparation increased mDNA yield by 200-fold but also decreased sensitivity for pathogen detection due to higher background noise. A recent stud...

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Literature Corpus work: 1d6bc953-efcb-5e8b-bfb1-84652df1ad8d
DOI: 10.1101/2025.11.06.25339688

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