Amplification-free Detection of Zoonotic Viruses Using Cas13 and Multiple CRISPR RNAs

Abstract excerpt

Zoonotic viruses such as hantaviruses and influenza A viruses present a threat to humans and livestock. There is thus a need for methods that are rapid, sensitive, and relatively cheap to detect infections with these pathogens early. Here we use an amplification-free CRISPR-Cas13-based assay, which is simple, cheap and field-deployable, to detect the presence or absence of genomic hantavirus or influenza A virus R...

Topics

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• Advanced biosensing and bioanalysis techniques
• CRISPR and Genetic Engineering
• Influenza Virus Research Studies
• Mosquito-borne diseases and control
• SARS-CoV-2 and COVID-19 Research
• SARS-CoV-2 detection and testing
• Viral Infections and Immunology Research
• Virology and Viral Diseases

Identifiers and source

Literature Corpus work

1193d1f8-3d6d-5a9e-9825-7bc35c46ad89

DOI

10.1101/2025.05.15.654407