Back to search

Article

Development of a long-read NGS workflow for improved characterization of fastidious respiratory mycoplasmas

2022-04-01

Abstract excerpt

Mycoplasmas are respiratory pathogens in humans and animals and due to their fastidious nature, they have been historically underdiagnosed. Lack of standardised diagnostic, typing and antimicrobial susceptibility testing methods makes clinical management and epidemiological studies challenging. The aim of this study was to develop a cost-effective and accurate sequencing workflow for genotypic characterization of...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
111a5a4f-728a-5f87-aba6-1bc1c47c6761
DOI
10.1101/2022.03.31.486658
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Development of a long-read NGS workflow for improved characterization of fastidious respiratory mycoplasmasDOI 10.1101/2022.03.31.486658
DNA Extraction Method Optimized for Nontuberculous Mycobacteria Long-Read Whole Genome SequencingSemantic proximity
Complete nontuberculous mycobacteria whole genomes using an optimized DNA extraction protocol for long-read sequencingSemantic proximity
A scalable, efficient, and safe method to prepare high quality DNA from mycobacteria and other challenging cellsSemantic proximity
A PCR protocol to establish standards for routine mycoplasma testing that by design detects over ninety percent of all known mycoplasma speciesSemantic proximity
Unveiling genome plasticity and a novel phage in <i>Mycoplasma felis</i> : genomic investigations of four feline isolatesSemantic proximity
Comprehensive subspecies identification of 175 nontuberculous mycobacteria species based on 7547 genomic profilesSemantic proximity
A mycobacterial DNA extraction protocol designed for resource limited settings generates high quality whole genome sequencingSemantic proximity
High throughput rapid amplicon sequencing for multilocus sequence typing of M. ovipneumoniae using DNA obtained from clinical samplesSemantic proximity
Mycoplasma genomics: tailoring the genome for minimal life requirements through reductive evolutionSemantic proximity
A systematic NGS-based approach for contaminant detection and functional inferenceSemantic proximity
Antimicrobial Susceptibility and Genomic Determinants of Resistance and Virulence in <i>Mycoplasma cynos</i> and <i>Mycoplasma felis</i>Semantic proximity
Target-enrichment sequencing yields valuable genomic data for difficult-to-culture bacteria of public health importanceSemantic proximity
Select a neighboring publication to make it the new centre.