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Article

Evaluation of A Single-reaction Method for Whole Genome Sequencing of Influenza A Virus using Next Generation Sequencing.

Biomedical and environmental sciences : BES - 1 Jan 2016

Zou Xiao Hui, Chen Wen Bing, Zhao Xiang, Zhu Wen Fei, Yang Lei, Wang Da Yan, Shu Yue Long

Abstract excerpt

OBJECTIVE: To evaluate a single-reaction genome ampliﬁcation method, the multisegment reverse transcription-PCR (M-RTPCR), for its sensitivity to full genome sequencing of influenza A virus, and the ability to differentiate mix-subtype virus, using the next generation sequencing (NGS) platform. METHODS: Virus genome copy was quantified and serially diluted to different titers, followed by amplification with the...

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Identifiers

PMID: 26822511
DOI: 10.3967/bes2016.004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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