Failure to achieve gene conversion with chimeric circular oligonucleotides: potentially misleading PCR artifacts observed.

doi:10.1089/oli.1.1998.8.531

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Article

Failure to achieve gene conversion with chimeric circular oligonucleotides: potentially misleading PCR artifacts observed.

Antisense & nucleic acid drug development - 1 Dec 1998

Zhang Z, Eriksson M, Falk G, Graff C, Presnell S C, Read M S, Nichols T C, Blombäck M, Anvret M

Abstract excerpt

Recently, a novel strategy for nucleotide exchange of target DNA using chimeric RNA/DNA oligonucleotides (CO) was reported. The CO can easily be transfected into cells, remain stable within the cells, and migrate to the nucleus. We have in this study used 42 similar constructs for targeting six d...

Topics

Animals
Artifacts
Base Sequence
Cell Line
Cells, Cultured
Chimera
DNA
Dogs
Gene Conversion
Humans
Mutation
Polymerase Chain Reaction
RNA

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