Failure to achieve gene conversion with chimeric circular oligonucleotides: potentially misleading PCR artifacts observed.

doi:10.1089/oli.1.1998.8.531

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Failure to achieve gene conversion with chimeric circular oligonucleotides: potentially misleading PCR artifacts observed.

Antisense & nucleic acid drug development - 1 Dec 1998

Zhang Z, Eriksson M, Falk G, Graff C, Presnell S C, Read M S, Nichols T C, Blombäck M, Anvret M

Abstract excerpt

Recently, a novel strategy for nucleotide exchange of target DNA using chimeric RNA/DNA oligonucleotides (CO) was reported. The CO can easily be transfected into cells, remain stable within the cells, and migrate to the nucleus. We have in this study used 42 similar constructs for targeting six different human and canine loci. A variety of cationic lipids, electroporation, and microinjection were used for...

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