Article
A spectroscopic and molecular dynamics study of native and of a mutant of Xenopus laevis Cu,Zn superoxide dismutase: mechanistic consequences of replacing four charged amino acids on the 'electrostatic' loop.
Biophysical chemistry - 14 Dec 1998
Falconi M, Venerini F, Desideri A
Abstract excerpt
Neutralisation by site-directed mutagenesis of four charged and highly conserved residues of the electrostatic loop of Cu,Zn superoxide dismutase from Xenopus laevis, involved in the electrostatic attraction of the substrate: Lys120-->Leu, Asp130-->Gln, Glu131-->Gln and Lys134-->Thr, gives rise t...
Topics
- Amino Acid Sequence
- Animals
- Chemical Phenomena
- Chemistry, Physical
- Hydrogen Bonding
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Recombinant Proteins
- Spectrophotometry
- Static Electricity
- Superoxide Dismutase
- Thermodynamics
- Xenopus laevis