Article
Expression of the bovine heart mitochondrial ADP/ATP carrier in yeast mitochondria: significantly enhanced expression by replacement of the N-terminal region of the bovine carrier by the corresponding regions of the yeast carriers.
Biochimica et biophysica acta - 5 Jan 1999
Hashimoto M, Shinohara Y, Majima E, Hatanaka T, Yamazaki N, Terada H
Abstract excerpt
To characterize the transport mechanism mediated by the mammalian mitochondrial ADP/ATP carrier (AAC), we tried to express bovine heart mitochondrial AAC (bhAAC) in Saccharomyces cerevisiae. The open reading frame of the bhAAC was introduced into the haploid strain WB-12, in which intrinsic AAC g...
Topics
- Amino Acid Sequence
- Animals
- Biological Transport
- Cattle
- Mitochondria
- Mitochondria, Heart
- Mitochondrial ADP, ATP Translocases
- Molecular Sequence Data
- Mutation
- Oxidative Phosphorylation
- Plasmids