Article
Temperature-dependent protein folding in vivo--lower growth temperature increases yield of two genetic variants of Xenopus laevis Cu,Zn superoxide dismutase in Escherichia coli.
Biochemical and biophysical research communications - 14 Aug 1992
Battistoni A, Carrì M T, Mazzetti A P, Rotilio G
Abstract excerpt
Two genetic variants of Xenopus laevis Cu,Zn superoxide dismutase, XSODA and XSODB, have been expressed in Escherichia coli by recombinant DNA techniques. Production of both proteins was obtained, although with different yields, XSODB being more abundant than XSODA in all the conditions tested. Lowering the temperature of growth was found to be a specific factor, decisive in obtaining quantitatively abundant,...
Topics
- Animals
- Cloning, Molecular
- Escherichia coli
- Gene Expression
- Genetic Variation
- Isoenzymes
- Molecular Weight
- Promoter Regions, Genetic
- Protein Conformation
- Protein Kinases