Article
PCR sequencing of the spike genes of geographically and chronologically distinct human coronaviruses 229E.
Journal of virological methods - 1 Nov 1998
Hays J P, Myint S H
Abstract excerpt
A reverse transcription nested PCR (RT-PCR) sequencing methodology was developed and used to generate sequence data from the spike genes of three geographically and chronologically distinct human coronaviruses 229E. These three coronaviruses were isolated originally from the USA in the 1960s (hum...
Topics
- Adult
- Amino Acid Sequence
- Child
- Coronavirus
- Coronavirus 229E, Human
- Genes, Viral
- Genetic Variation
- Ghana
- Humans
- Membrane Glycoproteins
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Spike Glycoprotein, Coronavirus
- Time Factors
- United Kingdom
- United States
- Viral Envelope Proteins