Article
Isolation and characterization of a mutant protoporphyrinogen oxidase gene from Chlamydomonas reinhardtii conferring resistance to porphyric herbicides.
Plant molecular biology - 1 Nov 1998
Randolph-Anderson B L, Sato R, Johnson A M, Harris E H, Hauser C R, Oeda K, Ishige F, Nishio S, Gillham N W, Boynton J E
Abstract excerpt
In plant and algal cells, inhibition of the enzyme protoporphyrinogen oxidase (Protox) by the N-phenyl heterocyclic herbicide S-23142 causes massive protoporphyrin IX accumulation, resulting in membrane deterioration and cell lethality in the light. We have identified a 40.4 kb genomic fragment encoding S-23142 resistance by using transformation to screen an indexed cosmid library made from nuclear DNA of the...
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