Article
Acquired mutations in phage lambda genes O or P that enable constitutive expression of a cryptic lambdaN+cI[Ts]cro- prophage in E. coli cells shifted from 30 degreesC to 42 degreesC, accompanied by loss of immlambda and Rex+ phenotypes and emergence of a non-immune exclusion-state.
Gene - 26 Nov 1998
Hayes S, Hayes C, Bull H J, Pelcher L A, Slavcev R A
Abstract excerpt
The majority of bacteria, which carry the N+-cI857[Ts]-cro--O+-P+ fragment of lambda genome, are killed when derepressed by shifting from 30 degreesC to 42 degreesC. Among rare survivors, we observed a proportion of colony-forming units (cfu) that retained the typical immlambda-immunity phenotype...
Topics
- Amino Acid Sequence
- Bacteriophage lambda
- Base Sequence
- Chromosome Mapping
- DNA-Binding Proteins
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Phenotype
- Repressor Proteins
- Temperature
- Viral Nonstructural Proteins
- Viral Proteins