Article
[Cellular microbiosensors for methanol and ethanol determination on the basis of pH-sensitive field transistors].
Ukrainskii biokhimicheskii zhurnal (1978) - 1 Jan 2000
Korpan Ia I, Gonchar M V, Soldatkin A P, Starodub N F, Sandrovskiĭ A K, Sibirnyĭ A A, El'skaia A V
Abstract excerpt
Cellular sensors for methanol and ethanol determination were developed using immobilized mutant cells of methylotrophic yeasts Hansenula polymorpha and Pichia pinus (able to extrude protons in the presence of alcohol) and pH-sensitive field effect transistors (pH-SFETs). The intact cells of yeasts were immobilized in Ca-alginate gel to obtain a biomembrane. The minimal detectable response was obtained to...
Topics
- Biosensing Techniques
- Ethanol
- Hydrogen-Ion Concentration
- Methanol
- Mutation
- Pichia
- Transistors, Electronic