Article
Long PCR analysis of human gamete mtDNA suggests defective mitochondrial maintenance in spermatozoa and supports the bottleneck theory for oocytes.
Biochemical and biophysical research communications - 18 Nov 1998
Reynier P, Chrétien M F, Savagner F, Larcher G, Rohmer V, Barrière P, Malthièry Y
Abstract excerpt
The long PCR and the Southern blot techniques were used to study mitochondrial DNA (mtDNA) in 94 sperm samples, and in 35 oocytes collected from 12 women. The sperm samples were classified in two sets: 37 samples from normal subjects, and 57 samples from patients with oligoasthenospermia. In both...
Topics
- Base Sequence
- Blotting, Southern
- DNA Primers
- DNA, Mitochondrial
- Female
- Gene Rearrangement
- Humans
- Male
- Models, Genetic
- Mutation
- Oligospermia
- Oocytes
- Oogenesis
- Polymerase Chain Reaction
- Sequence Deletion
- Spermatozoa