[Quantitative analysis of mRNA level by PCR method using single basemutated template as inner standard].

Article

[Quantitative analysis of mRNA level by PCR method using single basemutated template as inner standard].

Sheng li xue bao : [Acta physiologica Sinica] - 1 Apr 1997

Lu L M, Li H Y, Wang R, Yao T

Abstract excerpt

Using simple polymerase chain reaction (PCR) method, a single base changed mutant was generated, to which an EcoR I restriction site was added at a specific position of the primer template. The copies of the mutant could be quantified after amplification by PCR. Then the diluted mutant used as an...

Topics

Animals
DNA, Complementary
Liver
Male
Mutation
Polymerase Chain Reaction
RNA, Messenger
Rats
Rats, Sprague-Dawley
Receptors, Vasopressin

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