Article
The structure of serine hydroxymethyltransferase as modeled by homology and validated by site-directed mutagenesis.
Protein science : a publication of the Protein Society - 1 Sept 1998
Pascarella S, Angelaccio S, Contestabile R, Delle Fratte S, Di Salvo M, Bossa F
Abstract excerpt
We describe a model for the three-dimensional structure of E. coli serine hydroxymethyltransferase based on its sequence homology with other PLP enzymes of the alpha-family and whose tertiary structures are known. The model suggests that certain amino acid residues at the putative active site of...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- Escherichia coli
- Glycine Hydroxymethyltransferase
- Isoenzymes
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Structure, Tertiary
- Pyridoxal Phosphate
- Sequence Alignment