Article
Selection of EPS-deficient mutants (Exo-) from Rhizobiun huakuii 107 by region-directed Tn5 mutagenesis.
Chinese journal of biotechnology - 1 Jan 1998
Zhou B, Huang J, Su W, Song H
Abstract excerpt
pJB-B5 was a recombinant plasmid of pRK415 containing the 5.9 kb B5 fragment from exoR'-11. After pJB-B5 was mutagenized by MT614 (mal::Tn5), 10 plasmids TN1-1, TN1-12, TN2-2, TN2-3, TN3-1, TN4-1, TN9-1, TN10-1, TN13-1, and TN14-1 with different Tn5 insertions in the B5 fragment were constructed....
Topics
- DNA Transposable Elements
- Genetic Complementation Test
- Mutagenesis, Insertional
- Mutagenesis, Site-Directed
- Mutation
- Polysaccharides, Bacterial
- Recombination, Genetic
- Restriction Mapping
- Rhizobium
- Selection, Genetic