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Article

Selection of EPS-deficient mutants (Exo-) from Rhizobiun huakuii 107 by region-directed Tn5 mutagenesis.

Chinese journal of biotechnology - 1 Jan 1998

Zhou B, Huang J, Su W, Song H

Abstract excerpt

pJB-B5 was a recombinant plasmid of pRK415 containing the 5.9 kb B5 fragment from exoR'-11. After pJB-B5 was mutagenized by MT614 (mal::Tn5), 10 plasmids TN1-1, TN1-12, TN2-2, TN2-3, TN3-1, TN4-1, TN9-1, TN10-1, TN13-1, and TN14-1 with different Tn5 insertions in the B5 fragment were constructed. By conjugation of TN1-1, etc., into Rhizobium huakuii 107 containing the P-group plasmid pPH1JI which is incompatible...

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PMID: 9759544

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