Article
Genetic probing of the stalk segments associated with M2 and M3 of the plasma membrane H+-ATPase from Saccharomyces cerevisiae.
The Journal of biological chemistry - 9 Oct 1998
Soteropoulos P, Perlin D S
Abstract excerpt
The stalk region of the H+-ATPase from Saccharomyces cerevisiae has been proposed to play a role in coupling ATP hydrolysis to proton transport. Genetic probing was used to examine the role of stalk segments S2 and S3, associated with M2 and M3, respectively. Saturation mutagenesis was used to ex...
Topics
- Amino Acid Sequence
- Base Sequence
- Biological Transport
- Cell Membrane
- DNA Primers
- Glycine
- Isoleucine
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Proline
- Proton-Translocating ATPases
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid