Serines at the active site of 11 beta-hydroxysteroid dehydrogenase type I determine the rate of catalysis.

doi:10.1006/bbrc.1998.9313

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Article

Serines at the active site of 11 beta-hydroxysteroid dehydrogenase type I determine the rate of catalysis.

Biochemical and biophysical research communications - 18 Sept 1998

Obeyesekere V R, Trzeciak W H, Li K X, Krozowski Z S

Abstract excerpt

Short chain alcohol dehydrogenases have an invariant YXXXK motif at the active site. Database analysis of 116 superfamily members showed that 92% also contain a Serine or Threonine residue at the Y + 1 or Y + 3 positions, a pattern we previously described as the ST rule. In the present study we h...

Topics

11-beta-Hydroxysteroid Dehydrogenases
Animals
Binding Sites
Enzyme Activation
Hydroxysteroid Dehydrogenases
Mutagenesis, Site-Directed
Mutation
Peptides
Rats
Serine
Transfection

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