Article
The subsites structure of bovine pancreatic ribonuclease A accounts for the abnormal kinetic behavior with cytidine 2',3'-cyclic phosphate.
The Journal of biological chemistry - 2 Oct 1998
Moussaoui M, Nogués M V, Guasch A, Barman T, Travers F, Cuchillo C M
Abstract excerpt
The kinetics of the hydrolysis of cytidine 2',3'-cyclic phosphate (C>p) to 3'-CMP by ribonuclease A are multiphasic at high substrate concentrations. We have investigated these kinetics by determining 3'-CMP formation both spectrophotometrically and by a highly specific and quantitative chemical...
Topics
- Adenosine
- Animals
- Binding Sites
- Cattle
- Cytidine Monophosphate
- Cytosine Nucleotides
- Hydrolysis
- Kinetics
- Molecular Structure
- Mutation
- Oligoribonucleotides
- Poly C
- Protein Conformation
- Ribonuclease, Pancreatic
- Spectrophotometry
- Substrate Specificity
- Subtilisins