Article
Mutation of cis-proline 207 in mitochondrial creatine kinase to alanine leads to increased acid stability.
Protein engineering - 1 Jul 1998
Forstner M, Müller A, Rognan D, Kriechbaum M, Wallimann T
Abstract excerpt
We show that the mutation of an uncharged residue far from the active site to another uncharged residue can have effects on the active site without disturbing the overall structure of the protein. Cis-proline 207 of mitochondrial creatine kinase was mutated to alanine. The mutant showed a decreas...
Topics
- Creatine Kinase
- Escherichia coli
- Mitochondria
- Mutation
- Proline
- Protein Conformation
- X-Ray Diffraction