Article
Characterization and analysis of Chinese hamster ovary cell ERCC1 mutant alleles.
Mutagenesis - 1 Jul 1998
Rolig R L, Lowery M P, Adair G M, Nairn R S
Abstract excerpt
We isolated and characterized the ERCC1 coding sequence from three Chinese hamster ovary (CHO) parental (CHO-AA8, CHO-AT3-2 and CHO-9) and 10 ERCC1 mutant cell lines. Two general classes of mutations were observed: two mutant cell lines exhibited nucleotide additions or deletions to produce frame...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Western
- CHO Cells
- Cricetinae
- DNA-Binding Proteins
- Endonucleases
- Gene Expression Regulation
- Genetic Variation
- Molecular Sequence Data
- Mutation
- Proteins
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid