Article
Engineering an Mg2+ site to replace a structurally conserved arginine in the catalytic center of histidyl-tRNA synthetase by computer experiments.
Proteins - 15 Aug 1998
Arnez J G, Flanagan K, Moras D, Simonson T
Abstract excerpt
Histidyl-tRNA synthetase (HisRS) differs from other class II aminoacyl-tRNA synthetases (aaRS) in that it harbors an arginine at a position where the others bind a catalytic Mg2+ ion. In computer experiments, four mutants of HisRS from Escherichia coli were engineered by removing the arginine and...
Topics
- Adenosine Triphosphate
- Arginine
- Binding Sites
- Catalysis
- Computer Simulation
- Conserved Sequence
- Histidine-tRNA Ligase
- Magnesium
- Models, Molecular
- Mutation
- Protein Engineering
- Thermus thermophilus