Article
Isolation and characterization of strains defective in vacuolar ornithine permease in Neurospora crassa.
Fungal genetics and biology : FG & B - 1 Apr 1998
Keenan K A, Utzat C D, Zielinski T K
Abstract excerpt
Vacuolar uptake of ornithine and lysine was characterized in Neurospora crassa using a cupric ion permeabilization system. Michaelis constants were measured as 1.4 mM for lysine and 11.0 mM for ornithine, and maximal velocities were determined. Vacuolar lysine uptake was shown to be inhibited com...
Topics
- Amino Acid Transport Systems
- Arginine
- Biological Transport
- Copper
- Genes, Fungal
- Histidine
- Kinetics
- Lysine
- Membrane Transport Proteins
- Mutation
- Neurospora crassa
- Ornithine
- Substrate Specificity
- Vacuoles