Article
The role of histidine residues in the HXGH site of CTP:phosphocholine cytidylyltransferase in CTP binding and catalysis.
European journal of biochemistry - 1 Jul 1998
Veitch D P, Gilham D, Cornell R B
Abstract excerpt
The HXGH motif of CTP:phosphocholine cytidylyltransferase (CCT) is a unifying feature of the cytidylyltransferase family which has been proposed to function in binding of CTP and catalysis [Veitch, D. P. & Cornell, R. B. (1996) Biochemistry 35, 10743-10750]. Substitution of serine for Gly91 in th...
Topics
- Animals
- Binding Sites
- COS Cells
- Choline-Phosphate Cytidylyltransferase
- Cytidine Triphosphate
- Enzyme Stability
- Histidine
- Hydrogen Bonding
- Kinetics
- Liver
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Protein Denaturation
- Rats
- Transfection