Article
Determination of allele frequency from DNA pools using bovine trinucleotide microsatellites.
Animal biotechnology - 1 Jan 1998
Band M, Ron M
Abstract excerpt
Three sets of fluorescent labelled primers were used to amplify bovine trinucleotide microsatellites from DNA pools. DNA from 20 individuals were collected to create 3 pools differing in allele frequencies. Replicate mixes from each pool were used as template for PCR reactions. PCR products were...
Topics
- Alleles
- Animals
- Bias
- Binomial Distribution
- Cattle
- DNA
- Gene Frequency
- Gene Pool
- Genotype
- Linear Models
- Polymerase Chain Reaction
- Trinucleotide Repeats