Article
Determination of allele frequency from DNA pools using bovine trinucleotide microsatellites.
Animal biotechnology - 1 Jan 1998
Band M, Ron M
Abstract excerpt
Three sets of fluorescent labelled primers were used to amplify bovine trinucleotide microsatellites from DNA pools. DNA from 20 individuals were collected to create 3 pools differing in allele frequencies. Replicate mixes from each pool were used as template for PCR reactions. PCR products were separated and quantified on an automated DNA sequencer. Allele frequency estimates from pooled samples corrected for...
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