Article
Bovine hemoglobin alpha-globin chain polymorphism: primary structure determination of two new genetic variants by mass spectrometry and amino acid sequencing.
Biochimie - 1 Apr 1998
Scaloni A, Pieragostini E, Malorni A, Ferrara L, Di Luccia A
Abstract excerpt
The present work describes the biochemical procedures used to identify the cause of a quantitative and qualitative hemoglobin polymorphism found in Podolian cattle. First, to analyze the different phenotypes, isoelectric focusing (IEF) of hemoglobins and RP-HPLC of globin chains was carried out; secondly, to determine accurately the globin molecular masses, electrospray mass spectrometry was performed and finally...
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