PCR-based apolipoprotein E genotype analysis from archival fixed brain.

doi:10.1016/s0165-0270(98)00002-8

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Article

PCR-based apolipoprotein E genotype analysis from archival fixed brain.

Journal of neuroscience methods - 30 Apr 1998

Gioia L, Vogt L J, Freeman W M, Flood A, Vogt B A, Vrana K E

Abstract excerpt

A technique is described for determining the apolipoprotein E genotype (apo E; alleles epsilon2, epsilon3, or epsilon4) from tissues which have been fixed with 4-10% formaldehyde and archived. The procedure requires efficient extraction and exhaustive purification of DNA from the fixed tissue. Be...

Topics

Alzheimer Disease
Apolipoproteins E
Brain Chemistry
Cerebral Cortex
DNA
Fixatives
Genotype
Humans
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Tissue Fixation

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