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Article

Assessment of diagnostic quantitative fluorescent multiplex polymerase chain reaction assays performed on single cells.

Annals of human genetics - 1 Jan 1998

Sherlock J, Cirigliano V, Petrou M, Tutschek B, Adinolfi M

Abstract excerpt

We have refined polymerase chain reaction (PCR) assays for the detection of sickle cell anaemia, the delta F 508 deletion causing cystic fibrosis, and the IVS1-110 mutation leading to beta thalassaemia, allowing them to be successfully performed upon single cells using fluorescent primers. We have also assessed the possibility of detecting aneuploidies of chromosomes 13, 18 and 21 using a quantitative fluorescent...

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PMID: 9659974
DOI: 10.1046/j.1469-1809.1998.6210009.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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