Article
Assessment of diagnostic quantitative fluorescent multiplex polymerase chain reaction assays performed on single cells.
Annals of human genetics - 1 Jan 1998
Sherlock J, Cirigliano V, Petrou M, Tutschek B, Adinolfi M
Abstract excerpt
We have refined polymerase chain reaction (PCR) assays for the detection of sickle cell anaemia, the delta F 508 deletion causing cystic fibrosis, and the IVS1-110 mutation leading to beta thalassaemia, allowing them to be successfully performed upon single cells using fluorescent primers. We hav...
Topics
- Anemia, Hemolytic, Congenital
- Anemia, Sickle Cell
- Aneuploidy
- Cystic Fibrosis
- Female
- Fluorescence
- Hemoglobin, Sickle
- Heterozygote
- Humans
- Male
- Microsatellite Repeats
- Mutation
- Polymerase Chain Reaction
- Prenatal Diagnosis
- Sex Determination Analysis
- beta-Thalassemia