Article
In vitro selection of HIV-1 TAR variants by the Tat protein.
Journal of biotechnology - 15 Apr 1998
Marozzi A, Meneveri R, Giacca M, Gutierrez M I, Siccardi A G, Ginelli E
Abstract excerpt
Starting from a pool of 10(13) RNA sequences, we isolated a number of TAR RNA variants after nine rounds of selection by binding to recombinant Tat in vitro (SELEX procedure). Sequence analysis of part of the selected molecular species indicated that two TAR variants (clones A and B) were, respec...
Topics
- Base Sequence
- Binding Sites
- Biotechnology
- Cloning, Molecular
- DNA Primers
- Gene Products, tat
- Genetic Variation
- HIV Long Terminal Repeat
- HIV-1
- Humans
- In Vitro Techniques
- Molecular Sequence Data
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- RNA, Viral
- Transcriptional Activation
- Transfection
- tat Gene Products, Human Immunodeficiency Virus