A superrepressor mutant of the arginine repressor with a correctly predicted alteration of ligand binding specificity.

Abstract excerpt

Arginine biosynthesis in Escherichia coli is negatively regulated by the hexameric repressor protein ArgR and the corepressor L-arginine. L-Arginine binds to ArgR in the C-terminal domain of the repressor. Binding to operator DNA occurs in the N-terminal domain. The molecular structures of both d...

Topics

• Arginine
• Binding Sites
• DNA
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Mutation
• Protein Binding