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Article

Amplification and detection of the terminal 3' non-coding region of hepatitis C virus isolates.

Research in virology - 1 Jan 2000

Khorsi H, Salabi T, Castelain S, Jaillon O, Zawadzki P, Capron J P, Eb F, Wychowski C, Duverlie G

Abstract excerpt

A reverse transcription polymerase chain reaction (RT-PCR) assay was set up to amplify, from chronically infected patients, the recently discovered hepatitis C virus (HCV) 3'non-coding region (3'NCR). A panel of 149 samples was tested by RT-PCR for the 3'NCR. Two detection methods of amplified products were evaluated: ethidium bromide staining on 3% agarose gel electrophoresis and DNA enzyme immunoassay ("DEIA")....

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PMID: 9602506
DOI: 10.1016/s0923-2516(98)80087-3

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