Article
Requirement for ubiquinone downstream of cytochrome(s) b in the oxygen-terminated respiratory chains of Escherichia coli K-12 revealed using a null mutant allele of ubiCA.
Microbiology (Reading, England) - 1 Feb 1998
Balle Britta Sø, Poole Robert K
Abstract excerpt
An Escherichia coli knockout ubiCA mutant has been constructed using a gene replacement method and verified using both Southern hybridization and PCR. The mutant, which was unable to synthesize ubiquinone (Q), showed severely diminished growth yields aerobically but not anaerobically with either...
Topics
- Aerobiosis
- Alkyl and Aryl Transferases
- Alleles
- Anaerobiosis
- Cell Membrane
- Cloning, Molecular
- Cytochrome b Group
- DNA, Bacterial
- Electron Transport
- Escherichia coli
- Fumarates
- Lactic Acid
- NAD
- Nitrates
- Oxidation-Reduction
- Oxo-Acid-Lyases
- Oxygen
- Plasmids