Mutational studies with Atp12p, a protein required for assembly of the mitochondrial F1-ATPase in yeast. Identification of domains important for Atp12p function and oligomerization.

Abstract excerpt

The Atp12p protein of Saccharomyces cerevisiae is required for assembly of the F1 moiety of the mitochondrial ATP synthase. The current work has used mutant forms of Atp12p in an effort to learn about amino acids and/or domains that are important for the action of the protein. In one set of studies, the mutant atp12 genes were cloned and sequenced from 13 independent isolates of chemically mutagenized yeast. Of...

Topics

• Base Sequence
• Blotting, Western
• Chaperonins
• Fungal Proteins
• Mitochondria
• Mitochondrial Proteins
• Molecular Chaperones
• Mutation
• Oligomycins
• Oxygen Consumption
• Proton-Translocating ATPases