Article
Genes Preferentially Expressed in Bovine Oocytes Revealed by Subtractive and Suppressive Hybridization1
2 Jun 2005
Abstract excerpt
To isolate bovine oocyte marker genes, we performed suppressive and subtractive hybridization between oocytes and somatic tissues (i.e., intestine, lung, muscle, and cumulus cells). The subtracted library was characterized by sequencing 185 random clone inserts, representing 146 nonredundant genes. After Blast analysis within GenBank, 64% could be identified, 21% were homologous to unannotated expressed sequence...
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