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Simultaneous gene quantitation of multiple genes in individual bovine nuclear transfer blastocysts

1 Jan 2007

Abstract excerpt

During somatic cell nuclear transfer (NT), the transcriptional status of the donor cell has to be reprogrammed to reflect that of an embryo. We analysed the accuracy of this process by comparing transcript levels of four developmentally important genes (Oct4, Otx2, Ifitm3, GATA6), a gene involved in epigenetic regulation (Dnmt3a) and three housekeeping genes (beta-actin, beta-tubulin and GAPDH) in 21 NT...

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PMID: 17244749
DOI: 10.1530/rep.1.0966

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