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Article

Mutational analysis of the potato virus Y 5' untranslated region for alteration in translational enhancement in tobacco protoplasts.

Bioscience, biotechnology, and biochemistry - 1 Dec 1997

Yang L J, Hidaka M, Sonoda J, Masaki H, Uozumi T

Abstract excerpt

The 185 nucleotide 5' untranslated region (5'UTR) of potato virus Y ordinary strain (PVY-O) showed translation-enhancing activity on the beta-glucuronidase (GUS) gene in tobacco protoplasts. Mutational analysis of the 5'UTR was done to find sequence motifs necessary for the enhancement. Deletions within the 1-130 nucleotide region of 5'UTR stimulated the GUS expression in some cases, while the GUS activity...

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Identifiers

PMID: 9438996
DOI: 10.1271/bbb.61.2131

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