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Article

A cell surface tethered enzyme improves efficiency in gene-directed enzyme prodrug therapy.

Nature biotechnology - 1 Dec 1997

Marais R, Spooner R A, Stribbling S M, Light Y, Martin J, Springer C J

Abstract excerpt

The potential for expressing the bacterial enzyme carboxypeptidase G2 (CPG2) tethered to the outer surface of mammalian cells was examined for use in gene-directed enzyme prodrug therapy. The affinity of CPG2 for the substrate methotrexate was unaffected by three mutations required to prevent N-linked glycosylation. Breast carcinoma MDA MB 361 cells expressing CPG2 internally showed only a very modest increase in...

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PMID: 9415889
DOI: 10.1038/nbt1297-1373

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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