Article
Assembly of the N-dependent antitermination complex of phage lambda: NusA and RNA bind independently to different unfolded domains of the N protein.
Journal of molecular biology - 28 Nov 1997
Van Gilst M R, von Hippel P H
Abstract excerpt
The N protein of bacteriophage lambda activates expression of the delayed early genes of this phage by modifying RNA polymerase (RNAP) into a form that is resistant to termination signals. N binds to the boxB hairpin that forms in the nascent RNA transcript upon transcription of the nut regulatory element, and then interacts with RNAP by RNA looping. The binding of the N-boxB subassembly to the transcription...
Topics
- Bacterial Proteins
- Binding Sites
- Circular Dichroism
- DNA-Binding Proteins
- Escherichia coli Proteins
- Fluorescent Dyes
- Gene Expression Regulation, Viral
- Mutation
- Nucleic Acid Conformation
- Peptide Elongation Factors
- Peptide Fragments
- Protein Binding
- Protein Conformation